FIG. 1.

a) Average placental wet weights were determined for each litter (control, n = 8; restricted, n = 13; restricted + leptin, n = 12). Midsagittal sections were used to manually select and measure in ImageJ: cross-sectional area (b), cross-sectional area of each major placental zone (c), and the ratio of junctional zone:labyrinth zone area (d). Control, n = 7; restricted, n = 5; restricted + leptin, n = 6. Bars with different letters are significantly different (Tukey multiple comparison test). All measures shown (placental wet weight, placental area, labyrinth area, junctional zone area, and ratio of junctional:labyrinth zone) differed significantly among treatment groups (ANOVA, P < 0.05).