Effects of toluene on motor coordination, recognition memory and body temperature, and brain stimulation reward. (A) The latency to fall from the rotarod (n = 6), (B) the recognition index in the novel object recognition test (n = 6), (C) rectal temperature (n = 6), (D) ICSS thresholds (n = 9), and (E) ICSS response latency were determined after an injection of corn oil or toluene (250, 500, and 750 mg/kg). The data are expressed as mean ± SEM. Asterisks indicate a significant difference between toluene- and vehicle-treated mice (**p < 0.01, ***p < 0.001).