. 2011 Jun 22;1:125–129. doi: 10.1007/8904_2011_27

Table 1.

Oligonucleotides and reagents used for qRT-PCR

Gene	Primers	Probes	Fluorescent product
12S rRNA^a	CCACGGGAAACAGCAGTGAT	(6FAM)TGCCAGCCACCGCG(BHQ1)	6FAM/BlackHole Quencher
12S rRNA^a	CTATTGACTTGGGTTAATCGTGTGA	(6FAM)TGCCAGCCACCGCG(BHQ1)	6FAM/BlackHole Quencher
RNAseP^b	Taqman RNase P Control Reagents Kit. Part Number 4316844		VIC-TAMRA

^aOligonucleotides are located at positions 158 and 280 according to 12SrRNA sequence NC_012920; PCR amplification yields a 122 bp product

^bAccording to the manufacturer, Taqman technology uses amplicons around 100 bp in length