. 2011 Sep 27;3:71–77. doi: 10.1007/8904_2011_62

Supplementary Table 1.

Primer pairs and annealing temperature for the 14 prolidase sequenced exons

	Primers	Primer sequence (5′–3′)	Nt position NM_000285.3	Ta (°C)
Exon 2	ex2s	GTAGGTGGAGCTTGGGCAGCTTG	13974	55
Exon 2	ex2as	CAGCAGCACCATCACACACCTGC	14330	55
Exon 3	ex3s	TCGGTCTGAGCTGGCTCTCTAG	15639	52
Exon 3	ex3as	CAACCCAGCTCTCTCATCCCAC	15908	52
Exon 4	ex4s	GGCAGGCAGGAAGGTGGCCATG	25820	53
Exon 4	ex4as	GTGGCAGTGGAAGGAGAGGAAC	26018	53
Exon 5	ex5s	CCACGTCGCCTGTTCTAATGATC	33432	53
Exon 5	ex5as	CGACCTCTGCAGGAGAGGTGGC	33665	53
Exon 6	ex6s	CCTGTTCTTACCTGTCCTGAGCC	36736	53
Exon 6	ex6as	ACCTGCTAGTGAAGGTGGGAGTG	36940	53
Exon 7	ex7s	GCATCACGTGTGCATGTCTTG	48713	48
Exon 7	ex7as	GGTTCTGGGAATCTGCTTTCTG	48947	48
Exon 8	ex8s	CCAGTGCCTCCTGAAAGTCACTG	62779	62
Exon 8	ex8as	CTCTCGCCACACAGCAACACTGC	62943	62
Exon 9	ex9s	CATGTCTGTACCACTGCATGCAC	63730	54
Exon 9	ex9as	CACTGCGGCGTCTCATCTGTTAC	63967	54
Exon 10	ex10s	GCCTCACAGACTCACACTGAGAG	113099	54
Exon 10	ex10as	GGGCGTGTGAGTGAGCAAGTGTG	113412	54
Exon 11	ex11s	AGAGCCTGCTGCAGAAGAATGGGC	115092	57
Exon 11	ex11as	AGAAGGCACCACGCAGGCCGATAG	115377	57
Exon 12	ex12s	GTGGGAGACACCAGGTGGCAGTTC	124859	64
Exon 12	ex12as	CCGTTCCCTGCAGGCACCTGCCAC	125232	64
Exon 13	ex13s	CACAGCCAGTGTGAGGGCTTCAC	135319	54
Exon 13	ex13as	ACGCCTGCTGCCTCCTAGAGTCC	135672	54
Exon 14	ex14s	CCCTCACATGCCCATCTCTCCTG	138731	54
Exon 14	ex14as	CTCCACTGCAGCTGCAGACTCCT	139050	54
Exon 15	ex15s	CTGGCACATGAGGCTGCTCTATG	139362	54
Exon 15	ex15as	ATCAAATGCCGAAGCTGGGATCTG	139658	54