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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Nature. 2012 Jul 18;489(7416):447–451. doi: 10.1038/nature11352

Figure 2. HRDE-1 engages the Nrde nuclear RNAi pathway to direct multi-generational RNAi inheritance.

Figure 2

(a) pie-1::gfp::h2b fluorescence is shown. >98% of animals of each genotype exhibited phenotypes similar to that of image. (b) P0 animals expressing pie-1::gfp::h2b were exposed to gfp dsRNA. F1 progeny were exposed to nrde-2 or oma-1 dsRNA (n=3). (c) FLAG::NRDE-2 was precipitated with a-FLAG antibody and NRDE-2 co-precipitating oma-1 pre-mRNA was quantified with qRT-PCR using exon/intron primer sets. Data are expressed as ratio (+/−) oma-1 RNAi. (n=3, +/− s.e.m.). (d) The F1 progeny of oma-1 dsRNA-treated animals were subjected to H3K9me3 Chromatin Immunoprecipiation (ChIP). Co-precipitating oma-1 DNA was quantified with qRT-PCR. Data were normalized to co-precipitating eft-3 DNA and expressed as a ratio +/− oma-1 RNAi (1=no change). X-axis, 0 denotes predicted start codon of oma-1. (bp), base pair. rde-4 is required for RNAi (18) (n=3–4, +/− s.e.m.).