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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Nature. 2012 Jul 18;489(7416):447–451. doi: 10.1038/nature11352

Figure 4. The RNAi inheritance machinery promotes germline immortality.

Figure 4

(a) Animals of indicated genotypes were out-crossed to wild-type 2–4x and brood sizes scored across generations at 25°C. n=5, +/− s.e.m. Note: for unknown reasons, nrde-1 and nrde-4 mutants are Mrt at both 20°C and 25°C, but hrde-1 and nrde-2 mutants are only Mrt ~25°C (n=4). (b) hrde-1(−) animals were out-crossed 3x and (+/+) or (−/−) siblings were isolated. Gonads were isolated and stained with DAPI (DNA), and immuno-fluorescence was used to detect sperm (green) and oocytes (red) (see Methods) in F1 and F5 generations. Scale bar = 100µm.