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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Virology. 2012 Apr 21;428(2):112–120. doi: 10.1016/j.virol.2012.03.013

Fig. 1.

Fig. 1

Transient expression of latent genes do not induce lymphatic differentiation. (A) Constructs expressing individual latent viral genes were transiently transfected into TIME cells. 48 h post transfection, cell lysates were harvested and subjected to immunoblot analysis with the indicated antibodies. In addition, stable pooled TIME cells expressing LANA or a retroviral vector control were similarly analyzed. While expression of individual latent viral genes was confirmed by FLAG or LANA immunoblot, no viral gene activated STAT3 or induced VEGFR-3 expression. (B) TIME cells (2 × 105) were transduced with lentiviral preps of the indicated KSHV latent genes or pCGSW, a control lentivirus that expresses GFP. 72 hpi, cell lysates were harvested and subjected to immunoblot analysis with the indicated antibodies. TIME cells were mock or KSHV-infected and harvested 48 hpi for positive and negative controls respectively.