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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Virology. 2012 Apr 21;428(2):112–120. doi: 10.1016/j.virol.2012.03.013

Fig. 2.

Fig. 2

Expression of vIL-6 but not other lytic genes that activate AKT induce lymphatic differentiation. (A) TIME cells expressing the lytic viral proteins vIL-6, vOX2, HA-K3, or FLAG-K5 were created by retroviral transduction and antibiotic selection. Cell lysates were assayed by immunoblot analysis with the indicated antibodies. Stable vIL-6 expression induced VEGFR-3 expression and activated STAT3. (B) TIME cells (2 × 105) were transiently transduced with lentiviral preps of the indicated KSHV lytic genes or a control lentivirus (pCGSW). 72 hpi, cell lysates were harvested and subjected to immunoblot analysis with the indicated antibodies. TIME cells were mock or KSHV-infected and harvested 48 hpi for positive and negative controls respectively.