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. 2012 Nov 14;68(Pt 12):1427–1433. doi: 10.1107/S174430911204273X

Table 2. Results of in situ screening of RpiA crystallization hit conditions.

Crystal screen Well Well solution Outcome
Wizard I and II A6 20% PEG 3000, 0.1M citrate pH 5.5 Salt crystals
A8 2.0M ammonium sulfate, 0.1M citrate pH 5.5 No diffraction
G11 0.8M sodium phosphate/1.2M potassium phosphate, 0.1M acetate pH 4.5 Strong diffraction to 2.7 resolution, easily indexed and subsequently used for data collection
Morpheus A5 0.06M Divalents Mix, 0.1M Buffer System 2 pH 7.5, 30% PEG550MME_P20K Mix No diffraction
C1 0.09M NPS Mix, 0.1M Buffer System 1 pH 6.5, 30% PEG550MME_P20K Mix Diffraction spots observed to 6 resolution
D1 0.12M Alcohols Mix, 0.1M Buffer System 1 pH 6.5, 30% PEG550MME_P20K Mix Diffraction spots observed to 6 resolution
E1 0.12M Ethylene Glycols Mix, 0.1M Buffer System 1 pH 6.5, 30% PEG550MME_P20K Mix Diffraction spots observed to 6.5 resolution
G4 0.10M Carboxylic Acids Mix, 0.1M Buffer System 1 pH 6.5, 37.5% MPD_P1K_P3350 Mix Highly mosaic diffraction with spots observed to 3 resolution (harvesting of crystals unsuccessful, no further characterization)
H4 0.10M Amino Acids Mix, 0.1M Buffer System 1 pH 6.5, 37.5% MPD_P1K_P3350 Mix Diffraction spots observed to 6 resolution
H5 0.10M Amino Acids Mix, 0.1M Buffer System 2 pH 7.5, 30% PEG550MME_P20K Mix Diffraction spots observed to 9 resolution