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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Dev Biol. 2012 Oct 23;373(1):149–162. doi: 10.1016/j.ydbio.2012.10.017

Figure 6. Mef2C regulates R2R3 Hcn4 enhancer activity via a conserved Mef2 Site.

Figure 6

(A) Top, a conserved binding site for Mef2 was present within R3 as shown by an alignment between homologous regions of mouse, human, and opposum genomic DNA. Mismatches, shown in red, were not predicted to prevent Mef2 binding. Bottom, the Mef2 site in R3 was mutated at sites required for binding (mutations in red). (B) Transient R2R3-LacZ transgenic embryos bearing the Mef2 binding site mutation lost cardiac reporter activity. A founder bearing the unmutated R2R3 is shown for comparison (top left). Non-specific extracardiac ectopic activity confirmed transgene insertion into a permissive locus in the three founder embryos shown that bear the mutated Mef2 site in the context of R2R3.