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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Dev Biol. 2012 Oct 23;373(1):149–162. doi: 10.1016/j.ydbio.2012.10.017

Figure 8. HDAC Activity Regulates Hcn4 R2R3.

Figure 8

(A) MEF2C-VP16 was co-transfected with WT R3-Luc or MT R3-Luc. The Class II HDAC inhibitor MC1568 or dimethyl sulfoxide (DMSO) vehicle was added after transfection. Relative luciferase activity (RLA) was normalized to the value obtained with co-transfection of WT R3-Luc and empty pcDNA1 in the absence of MC1568. Means were compared with two-tailed t-tests (*, p<0.05; ***, p<0.001) (B,C) Wholemount bluo-gal staining of E11.5 R2R3-LacZ embryonic hearts that were cultured for 48 hours in 10 µM Trichostatin-A (TSA) or DMSO. (D,E). Immunhistochemistry for Hcn4 in cryosections of the left ventricle of TSA or DMSO exposed cultured E11.5 embryonic hearts. R2R3-LacZ embryonic hearts exposed to TSA showed an expansion of enhancer activity and increased ventricular Hcn4 expression.