Histograms of the particle rotations and shifts measured by motion tracking using movies. There is no significant difference in the distributions of the whole data set (1,915 particles, light gray) and the best 42% of the data (807 particles, dark gray). Particle motions are measured between averages of frames 1 – 4 and 5 – 8 (A, B), and between 1 – 4 and 13 – 16 (C, D). Each distribution is fitted with a Rayleigh distribution to determine its maximum λ (the median motion value).