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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: Structure. 2012 Sep 27;20(11):1823–1828. doi: 10.1016/j.str.2012.08.026

Figure 3.

Figure 3

Improvement in resolution with frame alignment. (A) The difference between the natural logarithm of the average structure factors F (dotted lines) of DLP reconstructions obtained with and without frame alignment, as a function of squared reciprocal resolution. The plots allow estimation by a linear fit of the B-factor reduction obtained with shift alignment (red line) and additional rotational alignment (purple line). (B) FSC curves for reconstructions of icosahedrally averaged reconstructions of DLP and rotavirus VP6 coat protein after additional 13-fold non-icosahedral averaging, calculated using data from all 16 movie frames. The dotted and solid lines correspond to DLP and VP6 reconstructions, respectively, while blue, red and purple correspond to reconstructions obtained without frame alignment, with shift alignment, and with both shift and rotational alignment, respectively.