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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: Structure. 2012 Sep 27;20(11):1823–1828. doi: 10.1016/j.str.2012.08.026

Figure 4.

Figure 4

Comparison of density maps. (A) α-helical region of the VP6 density calculated using images derived from movies by simple frame averaging without motion tracking. Density for some of the larger amino acid side chains is visible. (B) Same as in (A) but with shift alignment of the movie frames to reduce blurring in the images. The side-chain density is significantly stronger compared to (A). (C) Same as in (A) but with additional rotational alignment of the movie frames taken into account during 3D reconstruction. (D) Same as in (A) for the map published in 2008 (EMDB 1461). The crystal structure of VP6 (PDB ID: 3KZ4, McClain et al., 2010) is superimposed on the density in (A) – (D) to aid interpretation of the features. For accurate comparison, the maps in (A), (B) and (C) were scaled to match the rotationally averaged amplitude spectrum of the map in (D) and contoured at the same density threshold as in (D) using UCSF Chimera (Goddard et al., 2007).