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. 2012 Dec 1;23(23):4647–4661. doi: 10.1091/mbc.E12-04-0315

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© 2012 Koizumi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — N-WASP does not participate in RhoD-induced protrusion formation. (A) Neither N-WASP nor a dominant-negative N-WASP affects RhoD-induced protrusion formation. The 10T1/2 cells were cotransfected with mCherry–RhoD(G26V) (green) and EGFP–N-WASP or EGFP–N-WASP(ΔVCA) (magenta) and analyzed 48 h after the transfection. (B) Ratio of the cells extending thin and long protrusions in the analysis of (A). The values are mean ± SD of three independent experiments. *, p < 0.001 by t test compared with respective mock transfection (control). (C) Knockdown of N-WASP does not interfere with RhoD-induced protrusion formation. C2C12 myoblast clones stably expressing control and N-WASP siRNAs were transfected with EGFP–RhoD(G26V) and analyzed 48 h after the transfection. Scale bars: 10 μm. (D) Ratio of the cells extending thin and long protrusions in the analysis of (C). The values are mean ± SD of three independent experiments. *, p < 0.001 by t test compared with respective mock transfection (control).