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. 2012 Nov 29;8(11):e1003084. doi: 10.1371/journal.pgen.1003084

Figure 5. Phosphotransfer Assays with Phosphatase Mutants.

Figure 5

CrdS-WT or mutant proteins displaying reduced CrdA∼P phosphatase activity were tested for phosphotransfer capacity. CrdS proteins were allowed to reach maximal phosphorylation and then mixed with equimolar amounts of CrdA. Samples were collected at 0, 0.5, 1, 2, and 5 minutes and resolved by electrophoresis, imaged and quantified. Results indicate pmol of phosphorylated proteins (y-axis) for (▪) CrdA∼P and (♦) CrdS∼P over time (x-axis). (A) WT CrdS (B) V365A (C) R368A (D) L369A (E) N375A (F) P376A (G) P379A.