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. 2012 Nov 29;8(11):e1003084. doi: 10.1371/journal.pgen.1003084

Figure 7. Kinase and Phosphatase Assays with HisKA Proteins T. maritima HK853 and M. xanthus HK4262 and HK1190.

Figure 7

(a–c) In vitro kinase assays with purified protein. Each kinase was allowed to phosphorylate for 30 minutes and the amount of WT phosphorylated protein was set to 100%. (a) HK853 from T. maritima (b) HK4262 from M. xanthus (c) HK1190 from M. xanthus. (d–f) Phosphatase assays using acetyl-phosphate labeled response regulators. The amount of phosphorylated RR incubated in buffer alone was set to 100%. (d) T. maritima RR468 and HK853 (e) M. xanthus NtrC4261 and HK4262 (f) M. xanthus NtrC1189 and HK1190. Substitution mutants utilized here correspond to residues within the HisKA conserved sequence H-E/D-xx-T/N.