Table 1. Stability of CrdA∼P in the Presence of CrdS Mutant Proteins.
CrdS Protein | CrdA∼P Half Life (minutes) | SD | Reference |
- | 53.5 | 6.3 | [4] |
WT CrdS | 0.7 | 0.3 | [4] |
H371A | 2.6 | 0.5 | This Study |
E372A | 0.4 | 0.1 | This Study |
N375A | 47.6 | 3.2 | This Study |
E372A/N375A | 50.2 | 0.7 | This Study |
The half-life of CrdA∼P was calculated when incubated with CrdS WT and mutant proteins. Values shown are the mean ± standard error. CrdA∼P was generated using [32P] acetyl-phosphate and incubated with equimolar amounts of CrdS mutant proteins. The half-life of CrdA∼P incubated alone is indicated in the top row. Mutations affecting CrdS phosphatase activity display a corresponding increase in CrdA∼P half-life.