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. 2012 Nov 29;8(11):e1003084. doi: 10.1371/journal.pgen.1003084

Table 1. Stability of CrdA∼P in the Presence of CrdS Mutant Proteins.

CrdS Protein CrdA∼P Half Life (minutes) SD Reference
- 53.5 6.3 [4]
WT CrdS 0.7 0.3 [4]
H371A 2.6 0.5 This Study
E372A 0.4 0.1 This Study
N375A 47.6 3.2 This Study
E372A/N375A 50.2 0.7 This Study

The half-life of CrdA∼P was calculated when incubated with CrdS WT and mutant proteins. Values shown are the mean ± standard error. CrdA∼P was generated using [32P] acetyl-phosphate and incubated with equimolar amounts of CrdS mutant proteins. The half-life of CrdA∼P incubated alone is indicated in the top row. Mutations affecting CrdS phosphatase activity display a corresponding increase in CrdA∼P half-life.