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. 2012 Nov 29;8(11):e1003067. doi: 10.1371/journal.pgen.1003067

Figure 4. CELF4 protein is localized in soma and in neuronal projections.

Figure 4

A. Wildtype DIV14 cultured primary hippocampal neurons were examined for CELF4 protein localization by immunostaining with antibody against CELF4. Neuronal projections were stained with antibody against MAP2. Scale bar 20 µm. B–D. Sections from adult wildtype and Celf4 null mouse brains were examined for CELF4 protein localization by immunostaining with antibody against CELF4. In coronal cryosections, high CELF4 expression is seen in hippocampus, including the dentate gyrus (B), and in cortical layer V pyramidal neurons (D), which show CELF4 localization in soma and apical dendrites (D - top two panels). In sagittal vibratome sections, CELF4 localizes to soma and into dendrites in the CA3 region of the hippocampus as shown by colocalization with MAP2 (C – top two panels). The CELF4 antibody is specific as no signal above background is seen in Celf4 null hippocampal or cortical layer V cells (C,D - bottom two panels). Nuclei were stained with DAPI. Scale bar 20 µm.