Table 1. Molecular pathways and putative transcription regulatory networks affected during cardiac aging.

	N° of genes(1)	GO term enrichment(2)	Oxidative Stress		JNK pathway(5)	CisTargetX Analysis(6)
			MnSOD Transcriptome Enrichment(3)	Paraquat Transcriptome Enrichment(4)
Induced	635	defense response	147/1025	63/538	29/133	AP1/Jun (z = 6.78)
(cluster 2)		response to stress	p<10−20	p<2 10−5	p<10−7	Evi1 (z = 6.08)
		vesicle-mediated transport				GATA (z = 4.08)
		carbohydrate catabolic process
Repressed	472	cellular respiration	32/1305	37/611	nd	Vri/NFIL3 (z = 6.12)
(cluster 1)		electron transport chain	P = 0.3	P = 0.15
		hexose metabolic process

Overview of differential gene expression analysis between 10 days and 40 days adult cardiac tubes. Enrichment p values were calculated using a test based on hypergeometric distribution.

⁽¹⁾Statistically significantly deregulated genes between 10 and 40 day-old dissected hearts were determined using Limma software from normalized expression data (See Table S1 and S2).

⁽²⁾GO term enrichments were determined using the Flymine database (www.flymine.org) (see Table S5 for details).

⁽³⁾ [13]; See Table S6 for details.

⁽⁴⁾ [14]; See Table S7 for details.

⁽⁵⁾ [17], [18]; see Table S9 for details.

⁽⁶⁾Transcriptional regulatory network were predicted with cisTargetX (http://med.kuleuven.be/cme-mg/lng/cisTargetX/.) The z score of the most representative TF-binding motif is indicated for each motif family. (See Table S8 for details).