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. 2012 Nov 29;8(11):e1003073. doi: 10.1371/journal.pgen.1003073

Figure 1. Isolation of Spt5 mutations.

Figure 1

(A) Male flies carrying the [w+ GMroX1] transgene inserted at the 2R telomere (60F) have sectored pigmentation due to dosage compensation at the transgene. (B) Males heterozygous for mutations in Spt5 lose most red eye pigmentation due to reduced MSL complex activity. (C and D) Spt5 mutants have no effect on the PEV line In(1)wm4. (E) Genomic location of Spt5 and flanking deficiencies. (F) New Spt5 mutations failed to complement the previously reported Spt5MGE-3 allele [15]. (G) SPT5 domain features. SR, Serine/Arginine; NGN, N-terminal NusG; KOW, Kyrpides, Ouzounis, Woese light green oval indicates only partial match to consensus; RGG, arginine glycine glycine repeats; CTR, C-Terminal Repeats similar to RNAPII. Screen design and results are in Figure S1.