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. 2012 Nov 29;8(11):e1003073. doi: 10.1371/journal.pgen.1003073

Figure 4. MSL1 PEHE domain physically interacts with SPT5.

Figure 4

(A) MSL1 PEHE domain (aa 751–1039) was expressed as GST (Glutathione S-transferase) fusion protein. SPT5 fragments N, M, and C were expressed as MBP (Maltose binding Protein) fusion proteins [16]. (B) Purified SPT5-MBP fragments immobilized on amylose beads were allowed to interact with either GST-MSL1PEHE or GST. After washing, the recovered proteins were analyzed anti-GST Westerns. The membrane was stripped and reprobed to visualize the MBP fusion proteins. The middle input panel corresponds to 10% of the input GST proteins visualized with anti-GST antibodies.