Figure 2.

Sequence alignment showing the evolutionary conservation of LCAA in higher plants, mosses, eukaryotic algae, and cyanobacteria. The homologs from tobacco, Arabidopsis, and C. reinhardtii are nucleus encoded and therefore possess a putative chloroplast transit peptide at their N terminus. The LCAA homologs of the moss P. patens, the red alga P. purpurea, the brown alga F. vesiculosus, and P. chromatophora are all plastid encoded and therefore lack an N-terminal transit peptide. Additionally, sequences from two cyanobacteria (Synechococcus sp. WH8102 and N. punctiforme PCC 73102) were included. Identical amino acids are marked by stars, conserved substitutions by colons, and semiconserved substitutions by dots. The seed sequence of the Ycf54 domain (DUF2488) is underlined.