Figure 3.

Targeting of LCAA to chloroplasts. Fusions of GFP with the Arabidopsis and the tobacco LCAA genes were transiently transformed into tobacco protoplasts and expressed under the control of the strong constitutive cauliflower mosaic virus 35S promoter. Confocal laser scanning microscopy and overlays of GFP and chlorophyll a fluorescence revealed that both the tobacco and Arabidopsis proteins are targeted to the chloroplast, while the unfused GFP control is clearly retained in the cytosol.