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. 2012 Oct 17;160(4):1996–2006. doi: 10.1104/pp.112.204453

Figure 5.

Figure 5.

Transgenic manipulation of AIL1 affects adventitious root development. A, Percentage of rooted cuttings of P. tremula × P. tremuloides T89 (wild type), 35S:AIL1, and RNAi PtAIL1 lines. B, Percentage of rooted cuttings of P. tremula × P. alba 717-1B4 (wild type) and 35S:AIL transgenic lines (K4-5, K4-8, and K4-13). C, Number of adventitious roots per cutting in P. tremula × P. tremuloides T89 cuttings (wild type), 35S:AIL1, and RNAi line. D, Number of adventitious roots per cutting in P. tremula × P. alba 717-1B4 (wild type) and 35S:AIL transgenic lines (K4-5, K4-8, and K4-13). The curves show means and se. Student’s t and Fisher tests (P < 0.05) were used for analysis. Single asterisks indicate the day on which the differences became statistically significant in transgenic lines compared with wild-type plants, and double asterisks indicate a significant difference in the 35S:AIL1 K4-13 line compared with the 35S:AIL1 K4-8 line. E, Representative images of the adventitious root systems of T89 (wild type), 35S:AIL1, and RNAi line after 6 d of transfer to rooting induction medium. Bars = 1 cm. F, Representative poplar stem cross sections from 717-1B4 (wild type) and 35S:AIL1 (K4-8). Bars = 100 μm.