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. 2012 Oct 11;160(4):2125–2136. doi: 10.1104/pp.112.207027

Figure 2.

Figure 2.

Transcriptomic and proteomic profiles of SGF14c (Glyma05g29080) and SGF14l (Glyma08g12220) upon inoculation with B. japonicum. A, qRT-PCR results showing the relative transcript accumulation of each gene after normalization using the geometric mean of Actin and cons4 reference genes. In each histogram, the means of three biological replicates and three technical replicates are represented ± se (n = 3). The expression levels of each gene were measured in nodules and mock-inoculated roots at 10, 12, 16, 20, 24, and 32 dpi. The mock-inoculated roots were treated with water. Bars are as follows: black bars, SGF14c (Glyma05g29080); gray bars, SGF14l (Glyma08g12220). B, Specific antibodies (anti-SGF14cl) derived from SGF14c (Glyma05g29080) and SGF14l (Glyma08g12220) protein sequences (the polypeptide of the antibody is boxed). C, Western blot using anti-SGF14cl against proteins extracted from nodules and mock-inoculated roots at 10, 12, 16, 20, 24, and 32 dpi and the corresponding Coomassie blue-stained gel showing equal loading. The bottom band on the western blot belongs to SGF14c (Glyma05g29080), while the top band belongs to SGF14l (Glyma08g12220).