Table I. Protein identification of SGF14c (Glyma05g29080.1) and SGF14l (Glyma08g12220.1) from two-dimensional gel and mass spectrometry.
Boldface sequences are discriminative peptides for SGF14c and SGF14l. (ox)M, Oxidized Met; Mr(expt), relative molecular mass (experimental); Mr(calc), relative molecular mass (calculated); Δ, difference between experimental and calculated relative molecular masses.
| Start-End | Mr(expt) | Mr(calc) | Δ | Sequence | Mascot Score |
|---|---|---|---|---|---|
| Spot A Identificationa | |||||
| 6-15 | 1,253.69 | 1,253.64 | 0.0494 | K.ERENFVYVAK.L | 35 |
| 23-31 | 1,160.524 | 1,160.47 | 0.0513 | R.YEEMVEAMK.N | 44 |
| 36-45 | 1,200.682 | 1,200.64 | 0.0469 | K.LNVELTVEER.N | 70 |
| 46-53 | 892.5384 | 892.502 | 0.0366 | R.NLLSVGYK.N | 44 |
| 65-72 | 916.56 | 916.523 | 0.0371 | R.ILSSIEQK.E | 41 |
| 154-173 | 2,129.168 | 2,129.08 | 0.0928 | K.AYQLASTTAEAELASTHPIR.L | 137 |
| 200-218 | 2,174.091 | 2,173.99 | 0.1012 | K.QAFDEAISELDTLSEESYK.D | 110 |
| 219-228 | 1,188.705 | 1,188.65 | 0.0516 | K.DSTLIMQLLR.D | 69 |
| 219-228 | 1,204.698 | 1,204.65 | 0.0497 | K.DSTLI(ox)MQLLR.D | 61 |
| Spot B Identificationb | |||||
| 6-15 | 1,255.672 | 1,255.62 | 0.0528 | K.ERENFVYTAK.L | 48 |
| 36-45 | 1,200.671 | 1,200.64 | 0.0363 | K.LNVELTVEER.N | 52 |
| 46-53 | 892.5282 | 892.502 | 0.0264 | R.NLLSVGYK.N | 19 |
| 65-72 | 916.5614 | 916.523 | 0.0385 | R.ILSSIEQK.E | 31 |
| 219-228 | 1,204.68 | 1,204.65 | 0.0319 | K.DSTLI(ox)MQLLR.D | 46 |
a
Gene locus: Glyma05g29080.1; Mr: 29,160; pI: 4.8; coverage: 36%; annotation: Soybean 14-3-3 SGF14c.
b
Gene locus: Glyma08g12220.1; Mr: 29,475; pI: 4.9; coverage: 17%; annotation: Soybean 14-3-3 SGF14l.