Figure 7.
miR397 is related to copper homeostasis in L. japonicus and targets a copper-containing laccase. A, Predicted copper-responsive transcriptional activation motifs present in the 500-nucleotide (500nt) upstream genomic sequence of the lja-miR397 precursor. B and C, miR397 accumulation in nodules (B) and leaves (C) from plants grown at the indicated CuSO4 concentrations. U6 served as a loading control. Panels in B and C correspond to the same hybridization experiment. D, Validation of LjLACCASE10 as a miR397 target. Cleavage sites were determined by 5′ RACE analysis. The number of sequenced fragments ending at a respective site (out of total clones sequenced) is indicated. Predicted sites of endonucleolytic cleavage are indicated by arrows.