Energy and carbohydrate metabolism are enhanced after wounding. Phloem latex from untreated control and wounded plants at time points 3, 12, and 24 h after wounding were analyzed by GC-MS/MS. The results of three independent experiments with 11 biological replicates are shown. Box plots derived from ANOVA represent relative signal intensity ratios. Pyruvate and phosphate species (shaded) are central metabolites of the citric acid cycle (citrate and malate) and glycolysis/gluconeogenesis (Glc-6-P, Suc, and myoinositol). 3-hydroxypropionate is related to pyruvate (2-oxoproprionate) and lactate (2-hydroxypropionate) and is involved in energy metabolism, glycolysis, and Ala synthesis. C, Control. [See online article for color version of this figure.]