Table I. Wound-regulated proteins in phloem latex identified by isotope-coded protein labeling.
MW, Molecular mass in kD.
| Accessiona | MW | W3/Cb | W24/Cb | Description |
|---|---|---|---|---|
| Carbohydrate and energy metabolism | ||||
| 429162 | 31.1 | 2.21 | 2.49 | 14-3-3 protein 32-kD endonuclease |
| Cucsa.089980 | 35.9 | 0.72 | 0.58 | GDP-l-Fuc synthase |
| Cucsa.302670 | 33.6 | 0.33 | 0.33 | UDP-l-Rha synthase |
| Csa022482 | 59.2 | 0.33 | 0.43 | Glc-6-P dehydrogenase |
| Cucsa.170120 | 40.9 | 0.59 | 0.62 | SNF1-related protein kinase2 |
| 1743009 | 57.8 | 0.09 | 0.09 | SNF1-related protein kinase1 |
| Signaling, defense | ||||
| 10998336 | 48.1 | 3.25 | 4.06 | Silverleaf whitefly-induced protein1 |
| 113045960 | 17.9 | 1.09 | 1.69 | MAPK6 |
| 319439585 | 33.8 | 0.79 | 1.70 | Cyclin-dependent kinase A |
| 110748608 | 42.7 | 1.07 | 1.63 | NO3− stress-induced MAPK |
| 117573664 | 16.5 | 1.82 | 4.13 | 16-kD phloem protein1, PP16-1 |
| 1753099 | 95.3 | 1.06 | 2.09 | Phloem filament protein, PP1 |
| 508445 | 24.5 | 0.47 | 1.09 | Dimeric phloem-specific lectin, PP2 |
| 99906997 | 35.6 | 0.59 | 1.15 | Class III peroxidase precursor |
| 20453013 | 64.0 | 0.61 | 0.90 | Phloem calmodulin-like domain protein kinase |
| Cucsa.142500 | 16.9 | 0.83 | 0.56 | Calmodulin |
| 50262213 | 7.6 | 0.50 | 1.09 | Putative chymotrypsin protease inhibitor |
| Chaperones | ||||
| Csa001697 | 79.9 | 2.24 | 2.67 | HSP81 |
| Cucsa.142610 | 60.5 | 1.78 | 2.27 | Chaperonin-containing complex protein1 |
| Csa009634 | 59.0 | 1.43 | 2.00 | T-complex protein1 subunit ζ-like |
| 62728587 | 18.1 | 1.08 | 1.73 | Cyclophilin |
| Cucsa.106590 | 13.2 | 0.96 | 0.59 | Peptidyl-prolyl cis-trans-isomerase Pin1 |
| 51477394 | 63.8 | 0.57 | 1.21 | Protein disulfide isomerase (PDI)-like protein3 |
| Protein synthesis and degradation | ||||
| Cucsa.153230 | 67.1 | 3.23 | 1.38 | DEAD box RNA helicase |
| Cucsa.162420 | 53.5 | 1.42 | 1.72 | DEAD box RNA helicase |
| Cucsa.345110 | 85.1 | 2.04 | 2.42 | Elongation factor2-like |
| Cucsa.201940 | 16.6 | 2.40 | 1.66 | Ubiquitin-conjugating enzyme E2 |
| Cucsa.181800 | 48.0 | 2.11 | 2.64 | 26S protease subunit 6A-like |
| 81076307 | 16.5 | 1.63 | 2.00 | Putative translation initiation factor eIF-1A-like |
| 224110244 | 17.5 | 0.98 | 1.81 | Proteasome subunit α type 2 |
| 224085688 | 26.6 | 1.05 | 1.79 | Proteasome subunit α type 1 |
| Csa011709 | 86.7 | 1.58 | 1.77 | Cullin-1 |
| 147800085 | 66.7 | 0.53 | 1.26 | E3 ubiquitin ligase ARI7-like |
| 224095561 | 97.6 | 0.58 | 1.13 | 26S proteasome regulatory complex component |
| Cucsa.018140 | 51.2 | 0.60 | 0.78 | Eukaryotic translation initiation factor3 subunit 6N |
| Cucsa.342220 | 45.3 | 0.64 | 0.48 | COP9 signalosome subunit4-like |
| 307136429 | 25.5 | 0.41 | 0.34 | COP9 signalosome subunit7a |
| 298352997 | 17.2 | 0.31 | 0.36 | Putative ubiquitin-conjugating enzyme |
| Transport, cytoskeleton | ||||
| 495731 | 23.0 | 2.38 | 1.79 | Small ras-related |
| 6097869 | 13.8 | 0.93 | 3.52 | Actin |
| Csa010390 | 41.7 | 1.71 | 1.26 | Actin |
| Cucsa.053580 | 17.1 | 2.18 | 1.59 | Actin-depolymerizing factor |
| 157467219 | 18.5 | 2.44 | 1.73 | GTP-binding nuclear protein Ran3-like |
| 307135957 | 49.5 | 0.61 | 1.81 | Tubulin α-chain |
| 123192431 | 25.3 | 1.43 | 1.89 | Ran1 |
| 137460 | 68.8 | 0.83 | 1.73 | V-type proton ATPase catalytic subunit A |
| 255570599 | 96.4 | 0.53 | 1.01 | Importin β-1, putative |
| 51477379 | 99.8 | 0.52 | 1.26 | DRP, dynamin |
| Others | ||||
| Cucsa.328440 | 43.2 | 1.53 | 1.71 | S-Adenosyl-Met synthetase |
| 307135934 | 32.9 | 1.70 | 1.06 | Pyridoxal biosynthesis protein |
| 168049525 | 89.6 | 0.55 | 1.18 | Cell division control protein48, CDC48 |
a
Accession numbers from the National Center for Biotechnology Information, International Cucurbit Genomics Initiative (Csa identifiers), and the Joint Genome Institute (Cucsa identifiers). bC, W3, W24 indicate phloem latex from untreated control plants or wounded plants at 3- and 24-h time points.