Figure 4. IC₅₀ calculation after a 72-h period of exposure to currently used leishmanicidal drugs in infected peritoneal mouse macrophages with mCherry+L. major.

Drugs were added in a threefold dilution series, being the highest concentration 100, 2 and 500 µM for miltefosine, amphotericine B and paromomycin, respectively. IC₅₀ values were calculated from dose-response curves performed in triplicate and repeated twice after nonlinear fitting with the SigmaPlot program.