Figure 7. Effect of vaccination with Δhsp70-II Leishmania mutant on development of a BALB/c CL model produced by mCherry+L. major strain.
A) Top panel shows a non-infected animal (negative control) and the fluorescent signal emitted by hind footpads of animals infected with 2×105 metacyclic mCherry+L. major promastigotes at different time points. Bottom panel shows the footpads of animals immunized by intravenous administration of 107 Δhsp70-II metacyclic promastigotes per mouse four weeks before the challenge. Six mice in each group were imaged weekly. B) Parasite burdens in popliteal lymph nodes mean (LDAU/lymph node×105) ± SEM of six animals. Statistical differences were observed between groups; *, P<0.01 using de paired Student t test.