Table 1. Sequences of the primers and probes used in this study.
| Name (Location1) | Sequence (5′ to 3′)2 | Orientation | Length (bp)3 |
| Tgfbr1-f1 (847 to 873) | GAYAAYAARGAYAAYGGIACITGG | Sense | 359 |
| Tgfbr1-r1 (1183 to 1205) | GGIGCCATRTAICKYTTIGTICC | Antisense | |
| Tgfbr1-f2 (929 to 955) | TCAACCGTACTGTGGTCAGCATCTCCG | Sense | 91 |
| Tgfbr1-r2 (993 to 1019) | CCAATGATCTCCATGTGGAGGTGGGCG | Antisense | |
| Tgfbr1-f3 (219 to 242) | GATCTATATTCGAGTCCAAGAAGG | Sense | 1625 |
| Tgfbr1-r3 (1821 to 1843) | CATAAAGCTATTCATGTCTTCGG | Antisense | |
| Tgfbr1-f4 (1760 to 1784) | GAGGTGTCAGACTGATTTTCAGGAG | Sense | 101 |
| Tgfbr1-r4 (1836 to 1860) | ATGTCACAAAGGAAATTCATAAAGC | Antisense | |
| Tgfbr1-Pb (1803 to 1827) | TTGTAAGGAAGTTGGATACCGAACT | Probe | |
| Tgfbr1-f5 (335to 355) | ATCAACACCTGAGACCGACCT | Sense | 168 |
| Tgfbr1-r5 (480 to 502) | GGCACTGATGGTAGGCAAAGAAC | Antisense | |
| Actin-f | CAATCTACGAAGGTTATGCC | Sense | 186 |
| Actin-r | CCTGTTCAAAGTCAAGTGC | Antisense |
1
Location represents the position relative to the scallop Tgfbr1 translation start codon.
2
The bold and italics letter in the probe sequence indicates the position of the SNP.
3
The size of the amplicon.