Figure 7. LymP2X is widely expressed in Lymnaea CNS.

(A) RT-PCR using primers for LymP2X (primer pair 8 (Table 1) and β-tubulin (primer pair 9).+indicates PCR reactions on cDNA samples reverse transcribed from RNA prepared from the various ganglia indicated. – indicates negative control reactions where reverse transcriptase had been omitted from the cDNA synthesis reaction to control for genomic DNA contamination. M = molecular mass ladder (size in kb). (B) Quantitative PCR data showing the relative expression levels of LymP2X in various ganglia (normalised to pleural ganglia), n = 3 independent reactions for each sample.