Table 1. Oligonucleotide primers.
Name | Forward primer (5′-3′) | Name | Reverse primer (5′-3′) | |
1 | BlockA4For | TGTTGGGTCTTGGTCTACAAGAARGGNTAYCA | BlockFRev | CGATGTTGCCGAACTGGAANAYNGGRCA |
2 | BlockA2For | CCGCCGTGACCACCAARRTNAARGG | WCP1REV | TGGCATTTTGTCGTTCTCGRNNGGRCACCA |
3 | 5′ RACE Outer* | GCTGATGGCGATGAATGAACACTG | LymRACEout | CCTTTCAGGCGTTCACAATA |
4 | 5′ RACE Inner* | CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG | LymRACEin | CTTTGGTGGTCACAGCACTT |
5 | LymForB | TGAAAGGGACTATAATGCCA | ZAPREV2 | CCTCACTAAAGGGAACAAAA |
6 | LymKOZMTTfor | GCCGCCACCATGAATTTCAGAAATATTGATTGG | LymFULLrevin | AGGCTCGGGACTTATCAA |
7 | LymKOZMADfor | GCCGCCACCATGGCTGACCCAAAACACTG | LymFULLrevin | AGGCTCGGGACTTATCAA |
8 | LymP2Xfor | GGGATCGTCTTCGTGGTGA | LymP2Xrev | TGTCCTGAGGCGACTCTTCTT |
9 | β-tubulinfor | GAAATAGCACCGCCATCC | β-tubulinrev | CGCCTCTGTGAACTCCATCT |
10 | LyT7FORinsit | GAAATTAATACGACTCACTATAGGGACTATAATGCCAGGAGG | LyminsituREV | CCTTCAACAGATAGAGCACGATG |
11 | LyminsituFOR | GGGACTATAATGCCAGGAGG | LyT7REVinsit | GAAATTAATACGACTCACTATAGGGCC TTCAACAGATAGAGCACG |
Primer pairs 1−7 = cloning of LymP2X, * = Primers part of 5′RACE kit. 8−9 = primers used for RT-PCR and QRT-PCR. 10−11 = Primers used to generate templates for synthesis of sense (10) and antisense (11) cRNA probes used for in situ hybridization.