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. 2012 Nov 29;7(11):e50487. doi: 10.1371/journal.pone.0050487

Table 1. Oligonucleotide primers.

Name Forward primer (5′-3′) Name Reverse primer (5′-3′)
1 BlockA4For TGTTGGGTCTTGGTCTACAAGAARGGNTAYCA BlockFRev CGATGTTGCCGAACTGGAANAYNGGRCA
2 BlockA2For CCGCCGTGACCACCAARRTNAARGG WCP1REV TGGCATTTTGTCGTTCTCGRNNGGRCACCA
3 5′ RACE Outer* GCTGATGGCGATGAATGAACACTG LymRACEout CCTTTCAGGCGTTCACAATA
4 5′ RACE Inner* CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG LymRACEin CTTTGGTGGTCACAGCACTT
5 LymForB TGAAAGGGACTATAATGCCA ZAPREV2 CCTCACTAAAGGGAACAAAA
6 LymKOZMTTfor GCCGCCACCATGAATTTCAGAAATATTGATTGG LymFULLrevin AGGCTCGGGACTTATCAA
7 LymKOZMADfor GCCGCCACCATGGCTGACCCAAAACACTG LymFULLrevin AGGCTCGGGACTTATCAA
8 LymP2Xfor GGGATCGTCTTCGTGGTGA LymP2Xrev TGTCCTGAGGCGACTCTTCTT
9 β-tubulinfor GAAATAGCACCGCCATCC β-tubulinrev CGCCTCTGTGAACTCCATCT
10 LyT7FORinsit GAAATTAATACGACTCACTATAGGGACTATAATGCCAGGAGG LyminsituREV CCTTCAACAGATAGAGCACGATG
11 LyminsituFOR GGGACTATAATGCCAGGAGG LyT7REVinsit GAAATTAATACGACTCACTATAGGGCC TTCAACAGATAGAGCACG

Primer pairs 1−7 = cloning of LymP2X, * = Primers part of 5′RACE kit. 8−9 = primers used for RT-PCR and QRT-PCR. 10−11 = Primers used to generate templates for synthesis of sense (10) and antisense (11) cRNA probes used for in situ hybridization.