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. 2012 Nov 29;7(11):e50045. doi: 10.1371/journal.pone.0050045

Figure 5. The expression of genes involved in β-oxidation, fatty acid synthesis and gluconeogenesis in LH mutant mice.

Figure 5

Quantitative RT-PCR was used to determine changes in the gene expressions in LH mutant mice after 4 months of high fat diet. The columns represent the relative expression levels of muscular genes involved in the mitochondrial β-oxidation; CPT-1 and VLCAD and hepatic genes involved in the mitochondrial β-oxidation, fatty acid synthesis and gluconeogenesis; VLCAD, ACACA and PEPCK-C, respectively. For relative quantification of gene expression, the results were normalized using GADPH and β-actin as endogenous controls, and the expression of the wild type samples were set to 1. Results represent means with 95% confidence interval of 10 independently analyzed mice for each genotype. P values were calculated using unpaired heteroscedastic student t-test with one-tailed distribution. * p<0.05, ** p<0.01, *** p<0.001. Abbreviations: CPT-1 = carnitine palmitoyltransferase, VLCAD = very long chain acyl-CoA dehydrogenase, Acaca = acetyl-CoA carboxylase, PEPCK-C = phosphoenoylpyruvate carboxykinase.