Table 1. Distribution of recombinant adiponectin in MEF cell lysate and cell culture media.
Cells | Cell medium | Cell lysate | ||
ng/plateA | % of totalB | ng/plateA | % of totalB | |
MEF-wt | 497 | 66.0±3.2 | 260 | 34.0±3.2 |
MEF-LH3−/− knockout | 839 | 22.2±1.3 | 2928 | 77.8±1.3 |
Average of three experiments in which equal number of cells were used.
The results are calculated as a percentage of adiponectin from the total adiponectin on a cell culture plate (ng in lysate+ng in media measured by ELISA) and presented as average values ± S.D. from 3 different transfections.