Table II.
ESI-MS data for N-glycans of S3-RNase from L. peruvianum
| Glycan | Molecular
Mass
|
Composition | |||
|---|---|---|---|---|---|
| Native
Glycana
|
Per-O-Methylated Glycan
|
||||
| Measured | Calculated | Calculated | Measuredb | ||
| D | |||||
| S3a | 910.2 | 910.8 | 1149.3 | 1149.3 (60) | Hex3HexNAc2 |
| S3b | 1113.4 | 1114.0 | 1394.5 | 1394.2 (35) | Hex3HexNAc3 |
| S3c | NDc | – | 1554.7 | 1555.4 (5) | Hex3Pent1HexNAc3 |
a
Molecular masses of N-glycans were deduced from the molecular masses of the glycopeptides.
b
Numbers in parentheses indicate the percentage of individual pseudomolecular ions as estimated by ESI-MS.
c
ND, Not detected.