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. 2012 Nov 29;7(11):e50374. doi: 10.1371/journal.pone.0050374

Figure 4. RNAi effect on AaegGPRcal1 transcript and AaegGPRCAL1 expression in principal cells of female MTs.

Figure 4

(A) Relative quantification of AaegGPRcal1 transcript in the MTs five days after injection with AaegGPRcal1 dsRNA, EGFP dsRNA or water. Bars represent mean ± S.D.; 1 indicates the calibrator. Data were analyzed by ANOVA followed by Tukey multiple comparison test (common letter indicates not significantly different at 0.05 level). (B–D) Fluorescence microscopy analyses; images were obtained with the same exposure time (200 msec). Females injected with AaegGPRcal1 dsRNA exhibited lower AaegGPRCAL1 fluorescent signal intensity (B, yellow dashed line) by a factor of 3 than those injected with EGFP dsRNA (C, yellow solid line) or water (D, yellow solid line).