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. 2012 Nov 29;8(11):e1003052. doi: 10.1371/journal.ppat.1003052

Figure 1. Validation of pqs, PA3662 and hvnA genes.

Figure 1

Expression of chromosomal pqsA-lacZ (A), PA3662-lacZ (C) and hvnA-lacZ (E) fusions was monitored in the PAO1/pMMB67HE, PAO1ΔpprB/pMMB67HE, PAO1/pMMBpprB and PAO1ΔpprB/pMMBpprB strains. Data are expressed in Miller units and correspond to mean values (with error bars) obtained from three or six independent experiments. Statistical analysis was performed using a Welch (C and E) or a t-test (A) (*:<0.05, **:<0.01, ***:<0.001). EMSAs performed with the purified PprB-6His protein, at concentrations of 0 to 2.34 µM, and the putative pqs (B), PA3662 (D), and hvnA (F) promoter DNA regions.