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. 2012 Nov 29;8(11):e1003038. doi: 10.1371/journal.ppat.1003038

Table 1. Localization of NER proteins within the nucleus.

NER Protein Localization with respect to viral RCs in the nucleus
Fix First Procedure Extraction First Procedure
XPA Diffused throughout the nucleus Diffused throughout the nucleus
XPB* Associated Associated
XPC ND Associated
XPD* Associated Associated
XPF Diffused throughout the nucleus Associated
XPG Diffused throughout the nucleus Diffused throughout the nucleus
ERCC1 ND Associated
CSB ND Associated

HFFs were infected at an MOI of 5 for 48 h and harvested for IF analysis. Coverslips were treated using fix-first or extract-first conditions (as described in Materials and Methods).

*

indicates XPB and XPD showed tight association regardless of fixation conditions. ND = not determined.