Table 1. Localization of NER proteins within the nucleus.
NER Protein | Localization with respect to viral RCs in the nucleus | |
Fix First Procedure | Extraction First Procedure | |
XPA | Diffused throughout the nucleus | Diffused throughout the nucleus |
XPB* | Associated | Associated |
XPC | ND | Associated |
XPD* | Associated | Associated |
XPF | Diffused throughout the nucleus | Associated |
XPG | Diffused throughout the nucleus | Diffused throughout the nucleus |
ERCC1 | ND | Associated |
CSB | ND | Associated |
HFFs were infected at an MOI of 5 for 48 h and harvested for IF analysis. Coverslips were treated using fix-first or extract-first conditions (as described in Materials and Methods).
indicates XPB and XPD showed tight association regardless of fixation conditions. ND = not determined.