Figure 4. Polymorphisms found in the N- and C-terminal LRR units are critical for AvrL567 specificity.

A–E left panel: Schematic diagram of chimeric L5–L6 proteins. Domains from L6 are shaded white and domains from L5 are shaded grey. LRR units with polymorphic residues in the β-strand/β-turn structure (xxLxLxx motif) are marked with black bars. A–E right panel: Growth of yeast strain HF7c expressing chimeric L5–L6 proteins fused to the GAL4 activation domain and AvrL567-A, -D, and -J proteins fused to the GAL4 DNA-binding domain. Cultures were grown on selective media lacking tryptophan, leucine and histidine and scored after 4 days.