Figure 3. Hsp90 inhibitors disassociate LANA and Hsp90.

(A) BCBL-1 cells were harvested after treatment with 0.5 µM 17-DMAG for 0, 3, 6, 12, 24 hours separately, cells lysates were immunoprecipitated with rat monoclonal anti-LANA antibody, followed by immunoblotting analysis with mouse anti-Hsp90 and anti-LANA antibodies. Input samples were from supernatants of lysed cells. (B) BCBL-1 cells were harvested after treatment with 0, 10, 100 and 1000 µM AUY922 separately for 24 hours, cell lyses were used for immunoprecipitation assay with anti-Hsp90 and anti-p53 antibodies, followed by immunoblotting analysis with anti-LANA. (C) Hela cells were transfected with LANA vector treated with no drug (DMSO) or 0.1 µM AUY922 for 24 hours. Immunoprecipitation was performed with rat anti-LANA antibody, followed by immunoblotting analysis with anti-LANA and anti-Hsp90 antibodies; IgG was used as control.