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(A) Combination effect of temsirolimus and GSK690693 on cell proliferation determined by using the MTT assay. Cells were treated with the indicated concentrations of temsirolimus and GSK690693, and proliferation was determined 72 hours later. (B) Combination indices were calculated as described in Materials and Methods. Synergistic combination effect was found in higher concentrations of temsirolimus and GSK690693.
