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Inhibition of apoptosis or autophagy and its influence on the combination effect of GSK690693 and temsirolimus. Cells were pretreated with a pancaspase inhibitor, Z-VAD-FMK (50µM) and then treated with GSK690693 and temsirolimus for MTT analysis. On the other hand, cells were treated with GSK690693, temsirolimus, and an autophagy inhibitor, 3-methyladenine (3-MA; 250µM) for MTT analysis. *p<0.05, compared with non-treated control.
