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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Nutr Biochem. 2012 Aug 25;24(5):832–841. doi: 10.1016/j.jnutbio.2012.05.001

Figure 5.

Figure 5

Figure 5

Figure 5

RPMI 8866 integrin-independent cell adhesion is specifically induced by retinoids. (A) Various stimuli were cultured with RPMI 8866 tested to determine if their presence augments human B-cell adhesion to ADAM28 Dis-Fc. All vitamin or vitamin derivatives were incubated with RPMI 8866 cells for 72 hrs at 1 μM (B) RPMI 8866 B-cells were cultured in the presence of 1 μM 13-cis-RA or equimolar concentration of vehicle for 72 hrs and evaluated for adhesion on 3μg/mL sVCAM-Fc in the presence or absence of EDTA. For a positive control of integrin-dependent adhesion, 1 mM of Mn++ was added to the buffer. (C) Adhesion assays were repeated with RPMI 8866 cells stimulated with 13-cis-RA in the presence of the integrin inhibitors P1H4 (function blocking anti-α4) or EDTA. Displayed are the results obtained from three separate inhibition experiments each done in triplicate with 5 μg/mL of ADAM28 Dis-Fc ± SD.