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. 2012 Nov 30;3:262. doi: 10.3389/fpls.2012.00262

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Copyright © 2012 Wu, Oh, Kim, Schwartz, Imai, Yau, Clouse and Huber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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The E. coli proteins transphosphorylated by BRI1 covered a broad range in protein abundance. (A) Relative abundance of 62 of the 73 E. coli proteins transphosphorylated by BRI1 in situ. Blue diamonds are emPAI-derived copy number per cell (Ishihama et al., 2008) and red circles are values based on fluorescence measurements (Taniguchi et al., 2010) and the corresponding protein names are indicated on the top of the figure. The identity of the proteins quantified by emPAI method were: (1) rplV; (2) rpmD; (3) rplX; (4) crr; (5) rpsA; (6) ahpC; (7) gapA; (8) rpsB; (9) hupB; (10) rpsD; (11) fugA; (12) rpsC; (13) rplC; (14) tsf; (15) rplM; (16) hupA; (17) rpsL; (18) rpmE; (19) rpsE; (20) rplO; (21) rplE; (22) rpsM; (23) tig; (24) dnaK; (25) rpsF; (26) rplN; (27) rplJ; (28) grpE; (29) rpmA; (30) frr; (31) rpsJ; (32) rplK; (33) rpsl; (34) cspA; (35) rpsK; (36) sucB; (37) rpoA; (38) groS; (39) pnp; (40) rho; (41) rpoC; (42) juaA; (43) typA; (44) ybeD; (45) infB; (46) sucD; (47) pta; (48) ihfB; (49) yqjD; (50) rpoD; (51) ihfA; (52) aspA; (53) hslU; (54) ftsZ; (55) gatZ; (56) dnaJ; (57) accD; (58) lipA; (59) gatD; (60) tatA; (61) parB; (62) FimD. (B) Abundance of specific phosphopeptides, derived from E. coli proteins transphosphorylated by BRI1 in situ, was not related to the abundance of the parent protein. The spectral counts were the sum of four independent experiments, two with TiO₂ enrichment and two with IMAC (Fe²⁺) enrichment. The phosphopeptides with top spectral counts are annotated in the graph.