Table 2.
Identified autophosphorylation sites of Flag-BAK1 expressed in E.coli.
| Site | Counts | Mr (expt) | Mr (calc) | Expect | Peptide |
|---|---|---|---|---|---|
| S286 | 2 | 1430.4072 | 1430.6079 | 8.40E−05 | R.ELQVApSDNFSNK.N |
| S290 | 10 | 1430.4140 | 1430.6079 | 2.00E−06 | R.ELQVASDNFpSNK.N |
| S286 and S290 | 5 | 2063.6382 | 2063.9078 | 2.80E−10 | R.ELQVApSDNFpSNKNILGR.G |
| T324* | 6 | 2469.7507 | 2470.1022 | 4.20E−13 | R.pTQGGELQFQTEVEMISMAVHR.N |
| T324 | 5 | 2485.7923 | 2486.0971 | 2.00E−12 | R.pTQGGELQFQTEVE(ox)MISMAVHR.N |
| T324 | 2 | 2501.7946 | 2502.0920 | 2.60E−07 | R.pTQGGELQFQTEVE(ox)MIS(ox)MAVHR.N |
| T446 | 9 | 1078.1902 | 1078.4808 | 5.50E−03 | K.DpTHVTTAVR.G |
| T446 | 1 | 1728.6043 | 1728.7906 | 2.50E−04 | K.LMDYKDpTHVTTAVR.G |
| T449 | 31 | 1078.2848 | 1078.4808 | 4.60E−05 | K.DTHVpTTAVR.G |
| T450 | 2 | 1078.3472 | 1078.4808 | 2.00E−03 | K.DTHVTpTAVR.G |
| T446T449 | 4 | 1158.3110 | 1158.4472 | 3.20E−03 | K.DpTHVpTTAVR.G |
| T446T449 | 207 | 1808.5267 | 1808.7569 | 2.30E−09 | K.LMDYKDpTHVpTTAVR.G |
| T446T449 | 43 | 1824.4798 | 1824.7519 | 9.30E−09 | K.L(ox)MDYKDpTHVpTTAVR.G |
| T446T450 | 9 | 1158.2466 | 1158.4472 | 3.10E−04 | K.DpTHVTpTAVR.G |
| T446T450 | 6 | 1808.3242 | 1808.7569 | 1.20E−05 | K.LMDYKDpTHVTpTAVR.G |
| T446T450 | 1 | 1824.3166 | 1824.7519 | 3.70E−03 | K.L(ox)MDYKDpTHVTpTAVR.G |
| T449T450 | 11 | 1158.2642 | 1158.4472 | 1.30E−04 | K.DTHVpTpTAVR.G |
| T446T449T450 | 1 | 1238.2236 | 1238.4135 | 3.10E−02 | K.DpTHVpTpTAVR.G |
| T446T449T450 | 111 | 1888.5010 | 1888.7233 | 3.70E−08 | K.LMDYKDpTHVpTpTAVR.G |
| T446T449T450 | 1 | 1904.4894 | 1904.7182 | 1.10E−05 | K.LMDYKDpTHVpTpTAVR.G |
| Y443T449* | 5 | 1808.5414 | 1808.7569 | 3.00E−07 | K.LMDpYKDTHVpTTAVR.G |
| Y443T449 | 3 | 1824.2875 | 1824.7519 | 3.60E−03 | K.L(ox)MDpYKDTHVpTTAVR.G |
| Y443T449T450 | 17 | 1888.4788 | 1888.7233 | 1.10E−04 | K.LMDpYKDTHVpTpTAVR.G |
| Y443T446T450 | 1 | 1888.5172 | 1888.7233 | 2.90E−03 | K.LMDpYKDpTHVTpTAVR.G |
| T455 | 400 | 1622.3617 | 1622.7705 | 2.80E−10 | R.GpTIGHIAPEYLSTGK.S |
| T455S465* | 48 | 1702.5668 | 1702.7368 | 1.10E−07 | R.GpTIGHIAPEYLpSTGK.S |
| T455T466* | 37 | 1702.5156 | 1702.7368 | 1.20E−06 | R.GpTIGHIAPEYLSpTGK.S |
Tryptic peptides identified to contain phosphorylated residues (pS, pT, or pY) are shown in bold and sites not reported unambiguously earlier are identified with asterisks. All spectra were subject to manual verification. The experimental (expt) and calculated (calc) monisotopic masses are shown along with the expectation value reported in Mascot. The values for spectral counts for each identified peptide are the sums from three independent experiments. Oxidized methionine is identified as (ox)Met.