Fig. 1.

The ability of Fsk to induce rapid changes in LNCaP cell morphology is independent of de novo protein synthesis.

Panel A: LNCaP cells were pre-incubated for the indicated times in the presence of either vehicle (0.1% (v/v) EtOH) (open circles) or 100 μM Fsk (closed circles). Mean dendrite outgrowth was determined and results are presented as mean values ± SEM for n = 3 experiments. ***p < 0.001 vs. 0 h, ###p < 0.001 vs. vehicle at same time point.

Panel B: LNCaP cells were pre-incubated for 2 h in the presence of either vehicle (0.1% (v/v) DMSO) or 100 μM protein synthesis inhibitor emetine prior to stimulation with vehicle (0.1% (v/v) EtOH) or 10 μM Fsk for a further 1 h. Phase contrast images were captured immediately prior to the experiment (0 h) and following the 1 h stimulation with Fsk (post-stimulation).

Panel C: Mean dendrite outgrowth was assessed at 1 h post-stimulation as described previously. Results are presented as mean values ± SEM for n = 3 experiments. ***p < 0.001 vs. 0 h, ###p < 0.001 vs. vehicle at same time point.